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  • F4049 - Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse

F4049 Sigma

Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse



Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, FK-FO, FLAG Antibodies for Detection of FLAG-labeled Glycans,
biological source   mouse
antibody form   purified immunoglobulin
clone   M2, monoclonal
form   buffered aqueous solution
application(s)   direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype   IgG1
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C


Features and Benefits

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0.2, 1, 5×1 mg in poly bottle

Physical form

Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4


Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.

Browse additional application references in our FLAG® Literature portal.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

General description

Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibodies to Peptides and Fusion Tags

Unlike DNA and RNA, which can be targeted with complementary oligonucleotides, protein detection and purification usually relies upon specific antibodies. Although many are available commercially, no...
Leigh Gaskill
BioFiles 2008, 3.6, 14.
Keywords: Biofiles, Immunofluorescence, PAGE, Purification, Sequences, Western blot

Glycan Labeling

GlycoProfile™ Products for Metabolic Glycan Labeling FLAG® Antibodies for Detection of FLAG-labeled Glycans GlycoProfile™ Labeling Kits Fluorophores Chromophores Additional Reagents for Reductive Ami...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Aminations, Atomic absorption spectroscopy, Capillary electrophoresis, Cell proliferation, Chromatography, Degradations, Derivatizations, Electrophoresis, Gel electrophoresis, Glycosylations, High performance liquid chromatography, Ion Exchange, Mass spectrometry, Metabolism, Peptide synthesis, Phase transitions, Post translational modifications, Reductive aminations, Separation

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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