Synonym: Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse
|Related Categories||Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, Clone Index, FK-FO,|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|application(s)||direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone|
|shipped in||dry ice|
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Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4
Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.
Browse additional application references in our FLAG® Literature portal.
Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
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