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F4051 Sigma

Proteasome Fraction II from rabbit

5 mg/mL, Reticulocyte cell extract, solution

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Description

Application

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

Biochem/physiol Actions

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.

Protein fraction of a cell extract that binds to anion-exchange resin.

General description

Manufactured for Sigma by Boston Biochem., Inc.

Physical form

Solution in 25 mM HEPES, pH 7.6.

Price and Availability


DISCOVER Bioactive Small Molecules

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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