|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,|
|mol wt||mol wt 23.8 kDa|
Extinction Coefficient: E1% = 21.0 (280 nm)
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanili
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|Precautionary statements||P261-P305 + P351 + P338-P342 + P311|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Faceshields, Gloves|
|Hazard Codes (Europe)||Xn|
|Risk Statements (Europe)||36/37/38-42|
|Safety Statements (Europe)||22-24-26-36/37|
The spectrophotometric stop rate detemination (A280, Light path = 1 cm) is based on the following reaction:
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Variation in aspects of cysteine proteinase catalytic mechanism deduced by spectroscopic observation of dithioester intermediates, kinetic analysis and molecular dynamics simulations. Reid JD, Hussain S, Sreedharan SK, et al. Biochem. J. 357(Pt 2), 343-52, (2001)
A novel cysteine protease inhibitor with lectin activity from the epidermis of the Japanese eel Anguilla japonica. Saitoh E, Isemura S, Chiba A, et al. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 141(1), 103-9, (2005)
Molecular cloning and characterization of oryzacystatin-III, a novel member of phytocystatin in rice (Oryza sativa L. japonica). Ohtsubo S, Kobayashi H, Noro W, et al. J. Agric. Food Chem. 53(13), 5218-24, (2005)
Surface Plasmon Resonance Imaging biosensor for cystatin determination based on the application of bromelain, ficin and chymopapain. Gorodkiewicz E, Breczko J, and Sankiewicz A Folia Histochem. Cytobiol. 50(1), 130-6, (2012)
Production and characterization of two variants of human cystatin SA encoded by two alleles at the CST2 locus of the type 2 cystatin gene family. Saitoh E, Minaguchi K, and Ishibashi O Arch. Biochem. Biophys. 352(2), 199-206, (1998)
Ficin Liener, I. E., and Friedenson, B. Meth. Enzymol. 19, 261-273, (1970)
Glazer, A. N., and Smith, E. L. The Enzymes 3rdth ed., New York, NY , (1971), 538-542
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