• USA Home
  • F4229 - Monoclonal Anti-Fas (CD95/Apo-1)−FITC antibody produced in mouse

F4229 Sigma

Monoclonal Anti-Fas (CD95/Apo-1)−FITC antibody produced in mouse

clone DX2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Apo-1, Anti-CD95, Monoclonal Anti-Fas



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human
application(s)   flow cytometry: 1:50 using Cultured human Burkitt′s lymphoma Raji cells
clone   DX2, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... FAS(355)
biological source   mouse
usage   10 μL sufficient for 1 × 106 cells/0.1 mL staining (fluorescence intensity and maximum percentage positive similar to that obtained with saturating antibody levels)
conjugate   FITC conjugate



murine L cells transfected with a human Fas/CD95 cDNA.

General description

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these results in activation of the caspases. However, Fas-mediated death occurs much more rapidly than that triggered by TNFR1. Human Fas/CD95/Apo-1 is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.


Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.


Monoclonal Anti-Fas (CD95/Apo-1)-FITC antibody is suitable for flow cytometry at a dilution of 1:50 using cultured human Burkitt′s lymphoma Raji cells.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

APO-1/Fas(CD95) comprises of a death domain (DD) within the cytoplasmic region which triggers apoptosis upon binding of their cognate ligands. Once it is activated, APO-1/Fas(CD95) further aggregates its intracellular death domains which leads to the recruitment of two key signaling proteins followed by the formation of death-inducing signaling complex. These complex crosslinks through its C-terminal DD with APO-1/Fas receptors and engage caspase-8 via its N-terminal death effector domain (DED) to the DISC.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

WGK Germany  3
Protocols & Articles


Antibodies to Fas (CD95/Apo-1) and Fas Ligand (CD95L)

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing prot...
Keywords: Apoptosis, Ligands

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?