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F4274 Sigma

Fluorescein isothiocyanate isomer I

≥97.5% (HPLC)

Synonym: FITC, Fluorescein 5-isothiocyanate

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, E-F, Fluorescein and Derivatives, Fluorescence,
InChI Key   MHMNJMPURVTYEJ-UHFFFAOYSA-N
assay   ≥97.5% (HPLC)
form   powder
mp   >360 °C(lit.)
solubility   acetone: soluble10 mg/mL
fluorescence   λex 492 nm; λem 518 nm (green)
storage temp.   2-8°C

Description

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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