F4375 Sigma

Ficoll® PM 400

Type 400

Synonym: Polysucrose 400




Used for cell separation and organelle isolation.

Ficoll® PM 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque a density mixture used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. It may be used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-400 may be used in cell freeze-drying research.

Other Notes

A nonionic synthetic polymer of sucrose.


10, 25, 100, 500 g in poly bottle

Preparation Note

Concentrations of 50% (w/v) can be attained in water. Ficoll should be added slowly with constant stirring.
Sigma tests the solubility of Ficoll 400 at 1 g in 10 ml of deionized water yielding a clear to slightly hazy, colorless to faint yellow solution. Ficoll is stable in alkaline and neutral solutions. At pH values below 3, it is rapidly hydrolyzed, particularly at elevated temperatures. Ficoll can be sterilized by autoclaving at a neutral pH, at 110oC for 30 minutes. Strong oxidizing and reducing agents are to be avoided.

Features and Benefits

Because of the high molecular weight and low content of dialyzable material, Ficoll has a much lower permeability towards cell membranes than sucrose. Therefore, cells can be expected to collect at a lower density in Ficoll gradients than in sucrose gradients. Because of its low membrane permeability and low osmotic pressure, separations in Ficoll normally result in better preservation of cell function and morphology.

General description

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble. The most common application for Ficoll 400 is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells. Density ranges up to 1.2 g/ml can be attained. It has also been utilized in a variety of other applications.

Legal Information

Ficoll is a registered trademark of GE Healthcare

Price and Availability

OEM & Contract Manufacturing Services Available

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers


Set your institution to view full text papers.

Fractionation of rat ventricular nuclei. Jackowski, G, and Liew, C.C. Biochem. J. 188, 363-373, (1980)


Purification of gibberellic acid-induced lysosomes from wheat aleurone cells. Gibson, R.A., and Paleg, L.G. J. Cell Sci. 22, 413, (1976)


Preparation of extracts from yeast. Jazwinski, S.M Meth. Enzymol. 182, 154, (1990)


Superiority of visipaque (iodixanol)-controlled density gradient over Ficoll-400 in adult porcine islet purification. Min T, Yi L, Chao Z, Haitao Z, et al. Transplant. Proc. 42, 1825-1829, (2010)


Lymphocyte activation test for diagnosis of seronegative Brucellosis in humans. Almajid FM. Indian J. Pathol. Microbiol. 54, 775-781, (2011)


Resveratrol supplementation restores high-fat diet-induced insulin secretion dysfunction by increasing mitochondrial function in islet. Kong W, Chen LL, Zheng J, et al. Exp. Biol. Med. (Maywood.) 240(2), 220-9, (2015)


Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines. Kishimoto S, Ishihara M, Kanatani Y, et al. J. Tissue Eng. 2, doi: 10.1177/2041731411425419, (2011)

Human endothelial progenitor cells isolated from COPD patients are dysfunctional. Liu X, Xie C. Mol. Cell Biochem. 363(1-2), 53-63, (2011)


Soluble form of the endothelial adhesion molecule CD146 binds preferentially CD16+ monocytes. Garibaldi S, Barisione C, Ghigliotti G, et al. Mol. Biol. Rep. 39(6), 6745-52, (2012)


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