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F4424 Sigma

Monoclonal Anti-Fas (CD95/Apo-1) antibody produced in mouse

clone DX2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Apo-1, Anti-CD95, Monoclonal Anti-Fas

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human
application(s)   flow cytometry: 4-20 μg/mL using cultured human Burkitt’s lymphoma Raji cells
  microarray: suitable
clone   DX2, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FAS(355)
biological source   mouse
usage   10 μL sufficient for 1 × 106 cells fluorescence intensity and maximum percentage positive similar to that obtained with saturating antibody levels
conjugate   unconjugated

Description

Immunogen

murine L cells transfected with a human Fas/CD95 cDNA.

General description

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these results in activation of the caspases. However, Fas-mediated death occurs much more rapidly than that triggered by TNFR1. Human Fas/CD95/Apo-1 is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa).

Physical form

Solution from a bioreactor culture supernatant in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Fas (CD95/Apo-1) antibody is suitable for apoptosis treatment of Jurkat cells to study the contribution of channel type into the net K+ flux. It is also suitable for flow cytometry at a concentration of 4-20μg/mL using cultured human Burkitt’s lymphoma Raji cells.

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Biochem/physiol Actions

Human CD95/Fas/Apo-1 antigen is a single transmembrane glycoprotein receptor of 325 amino acids (45-48 kDa) which activate cell apoptosis. The action of Fas is mediated via FADD (Fas-associated death domain)/ MORT1, an adapter protein that has a death domain at its C-terminus and binds to the cytoplasmic death domain of Fas. APO-1/Fas(CD95) comprises of a death domain (DD) within the cytoplasmic region which triggers apoptosis upon binding of their cognate ligands. Once it is activated, APO-1/Fas(CD95) further aggregates its intracellular death domains which leads to the recruitment of two key signaling proteins followed by the formation of death-inducing signaling complex. These complex crosslinks through its C-terminal DD with APO-1/Fas receptors and engage caspase-8 via its N-terminal death effector domain (DED) to the DISC.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Fas (CD95/Apo-1) and Fas Ligand (CD95L)

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing prot...
Keywords: Apoptosis, Ligands

esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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Peer-Reviewed Papers
15

References

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