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F4637 Sigma

Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, HU-HZ, Human Polyvalent Ig Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   ANA-indirect immunofluorescence: 1:128
  direct immunofluorescence: 1:80
conjugate   FITC conjugate
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

Purified human IgA, IgG, and IgM

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a working antibody dilution of 1:128. For anti-nuclear antibody assay of acetone-fixed rat liver cells, a minimum dilution of 1:128 is recommended. For direct labeling of human tonsil sections, a dilution of 1:80 may be used. This antibody was used to stain C2C12 cell line derived from mouse myoblasts at a dilution of 1:100

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Purified human IgA, IgG and IgM are used to develop anti-sera in rabbits. The IgG fraction of each anti-sera is purified and conjugated to Fluorescein Isothiocyanate (FITC), in an alkaline reaction. Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody specifically recognizes human IgA, IgG and IgM.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
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Protocols & Articles

Articles

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