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F4639 Sigma

Monoclonal Anti-Fibrinogen antibody produced in mouse

clone FG-21, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood, Antibodies to Clotting Factors,
species reactivity   baboon, human
application(s)   indirect ELISA: 1:4,000
clone   FG-21, monoclonal
antibody form   ascites fluid
isotype   IgG2a
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FGA(2243), FGB(2244), FGG(2266)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified haptoglobin from pooled human plasma containing the three major haptotypes.

General description

Fibrinogen, is a thrombin-coagulable soluble plasma 340kDa glycoprotein, composed of paired sets of three subunits i.e. α, β, γ. It plays a crucial role in protecting the vascular network against the loss of blood after tissue injury. Among three subunits, β and γ subunits contain one N-glycosylation site, which is occupied by a biantennary N-glycan. It contains three pairs of disulfide bonded chains called α, β, γ which further folded into four structural domains: the D, E, connector and the COOH-terminal region of the Aα chain.

Specificity

The antibody is specific for human fibrinogen and recognizes an epitope sensitive to reduction commonly used in immunoblotting assays. It also reacts with synthetic human fibrinopeptides A and B, fibrinogen related peptides and baboon fibrinogen. No cross-reactivity with bovine, pig, sheep, horse, dog, goat, rabbit, guinea pig, or rat is observed.

Application

Monoclonal Anti-Fibrinogen antibody is suitable for SDS-PAGE and western ligand blot analysis of E. coli lysates containing recombinant y chains. It is also suitable for indirect ELISA at a dilution of 1:4,000.

Features and Benefits

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Biochem/physiol Actions

Plasmin attacks the Aα chain COOH domain to produce the heterogeneous fragment X. After a series of degradation, it ended with terminal digestion products: fragments D and E which represent the major globular domains in fibrinogen. Mutations in this gene leads to several disorders including hypofibrinogenemia, afibrinogenemia.

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