Fibronectin from bovine plasma
powder, BioReagent, suitable for cell culture
DOWNLOAD MSDS (PDF)Synonym: CIG, Cold insoluble globulin
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CAS Number 86088-83-7
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EC Number 289-149-2
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MDL number MFCD00131062
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Popular Documents: Product Information Sheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | 2D Cell Culture, Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Application Index, Attachment Factors, |
| product line | BioReagent |
| form | powder |
| surface coverage | 1‑5 μg/cm2 |
| solubility | H2O: soluble1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.) |
| suitability | suitable for cell culture |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Analysis Note
Protein homogeneity is evaluated by immunoelectrophoresis.
Application
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Bovine plasma fibronectin has been shown to stimulate the proliferation of rat osteoblasts in vitro; to induce odontoblast polarization and to regulae the differentiation of human dental pulp cells into odontoblasts.
Caution
Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Reconstitution
Store reconstituted solution in working aliquots at -20°C or lower.
Physical form
Lyophilized from 0.05 M Tris buffered saline, pH 7.5
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