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F4759 Sigma

Fibronectin from bovine plasma

powder, BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin




Cellular fibronectin is an adhesion glycoprotein of the ECM,k existing as a dimer of an A chain and the B chain containing the type III connecting segment (IIIcs) region. Cellular fibronectin is different from plasma fibronectin by the presence of additional polypeptide segments and in an altered morphology of transformed cells and hemagglutination. Different forms of fibronectin can be generated from tissue specific splicing of fibronectin mRNA transcribed from a single gene.


This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives. Bovine plasma fibronectin has been shown to stimulate the proliferation of rat osteoblasts in vitro, to induce odontoblast polarization and to regulae the differentiation of human dental pulp cells into odontoblasts.

Fibronectin from bovine plasma is used for the following applications:
• Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)1
• Cell movement assays (culture plates were coated with fibronectin)2
• Cell spreading and immunofluorescence3
• Protein specificity4
• Integration of sensor films into microfluidic chips5
• Histological and Immunohistochemical (IHC) analyses6
• Used in primary cell cultures from patients with lung carcinoma7

Biochem/physiol Actions

Domains of fibronectin bind with collagen, fibrin, heparin, and specific cell membrane receptors. The most notable domain, Arg-Gly-Asp can be recognized by integrins and mediates cell adhesion. Fibronectin is also widely involved in interactions such as the attachment and migration of many cell types, cytoskeletal assembly, tyrosine phosphorylation, and metastasis.


As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions, Tools


Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Vicki Caligur
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Events, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation

Peer-Reviewed Papers


Set your institution to view full text papers.

1. Macrophage functions are regulated by the substratum of murine decidual stromal cells. Redline RW, McKay DB, Vazquez MA, et al. J. Clin. Invest. 85(6), 1951-8, (1990)


2. Thrombospondin-1 is a transcriptional repression target of PRMT6. Michaud-Levesque J and Richard S J. Biol. Chem. 284(32), 21338-46, (2009)


3. An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization. Huot ME, Brown CM, Lamarche-Vane N, et al. Mol. Cell. Biol. 29(7), 1933-43, (2009)


4. Isolation of a bovine plasma fibronectin-containing complex which inhibits the expression of bovine leukemia virus p24. van den Heuvel MJ, Jefferson BJ, and Jacobs RM J. Virol. 79(13), 8164-70, (2005)


5. Microfluidic oxygen imaging using integrated optical sensor layers and a color camera. Ungerböck B, Charwat V, Ertl P, et al. Lab Chip 13(8), 1593-601, (2013)


6. Evaluation of silk biomaterials in combination with extracellular matrix coatings for bladder tissue engineering with primary and pluripotent cells. Franck D, Gil ES, Adam RM, et al. PLoS ONE 8(2), e56237, (2013)


7. Decoy receptor-2 small interfering RNA (siRNA) strategy employing three different siRNA constructs in combination defeats adenovirus-transferred tumor necrosis factor-related apoptosis-inducing ligand resistance in lung cancer cells. Aydin C, Sanlioglu AD, Karacay B, et al. Hum. Gene Ther. 18(1), 39-50, (2007)


MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons. Richner M, Victor MB, Liu Y, et al. Nat. Protoc. 10(10), 1543-55, (2015)


An intron polymorphism of the fibronectin gene is associated with end-stage knee osteoarthritis in a Han Chinese population: two independent case-control studies. Yang HY, Su SL, Peng YJ, et al. BMC Musculoskelet. Disord. 15, 173, (2014)


Stoichiometry of molecular complexes at adhesions in living cells. Digman MA, Wiseman PW, Choi C, et al. Proc. Natl. Acad. Sci. U. S. A. 106(7), 2170-5, (2009)


Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method. Michelle A. Digman et al Biophys. J. 96, 707-16, (2009)


The deubiquitinases USP33 and USP20 coordinate β2 adrenergic receptor recycling and resensitization. Magali Berthouze et al EMBO J. 28, 1684-96, (2009)


Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury. De Laporte L Biomaterials 30(12), 2361-8, (2009)


High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells. Berginski ME, Vitriol EA, Hahn KM, et al. PLoS ONE 6(7), e22025, (2011)


TGF-β mediates PTEN suppression and cell motility through calcium-dependent PKC-α activation in pancreatic cancer cells. Jimmy Y. C. Chow et al Am. J. Physiol. Gastrointest. Liver Physiol. 294, G899-905, (2009)


Regulation of EphB2 activation and cell repulsion by feedback control of the MAPK pathway. Alexei Poliakov et al J. Cell Biol. 183, 933-47, (2008)


Integrin alpha1beta1 regulates matrix metalloproteinases via P38 mitogen-activated protein kinase in mesangial cells: implications for Alport syndrome. Cosgrove, D Am. J. Pathol. 172(3), 761-73, (2008)


Inductive specification and axonal orientation of spinal neurons mediated by divergent bone morphogenetic protein signaling pathways. Jeanette C Perron et al Neural Dev. 6, 36, (2011)


Overexpression of cell surface cytokeratin 8 in multidrug-resistant MCF-7/MX cells enhances cell adhesion to the extracellular matrix. Fang Liu et al Neoplasia 10, 1275-84, (2008)


Characterization of G2L3 (GAS2-like 3), a new microtubule- and actin-binding protein related to spectraplakins. Stroud MJ, Kammerer RA, and Ballestrem C J. Biol. Chem. 286(28), 24987-95, (2011)


Astrocyte-specific genes are generally demethylated in neural precursor cells prior to astrocytic differentiation. Hatada I PLoS ONE 3(9), e3189, (2008)


Smad4-dependent pathways control basement membrane deposition and endodermal cell migration at early stages of mouse development. Costello I, Biondi CA, Taylor JM, et al. BMC Dev. Biol. 9, 54, (2009)


OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier. Lucia Pulzova et al Sci. Rep. 1, 86, (2011)


Direct interaction of tumor suppressor CEACAM1 with beta catenin: identification of key residues in the long cytoplasmic domain. Jin L Exp. Biol. Med. (Maywood.) 233(7), 849-59, (2008)


Fibronectin and fibronectin fragments. Akiyama, S. and Yamada, K., Haralson, M. and Hassell, J.R., ed. Extracellular Matrix: A Practical Approach New York , (1995), 183

Growth factors and other nonnutrient factors. Butler, M., Butler, M and Dawson, M.,ed. Cell Culture Labfax Oxford, England , (1992), 160

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