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F4759 Sigma

Fibronectin from bovine plasma

powder, BioReagent, suitable for cell culture

Synonym: CIG, Cold insoluble globulin

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Description

Components

Cellular fibronectin is an adhesion glycoprotein of the ECM,k existing as a dimer of an A chain and the B chain containing the type III connecting segment (IIIcs) region. Cellular fibronectin is different from plasma fibronectin by the presence of additional polypeptide segments and in an altered morphology of transformed cells and hemagglutination. Different forms of fibronectin can be generated from tissue specific splicing of fibronectin mRNA transcribed from a single gene.

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives. Bovine plasma fibronectin has been shown to stimulate the proliferation of rat osteoblasts in vitro, to induce odontoblast polarization and to regulae the differentiation of human dental pulp cells into odontoblasts.

Fibronectin from bovine plasma is used for the following applications:
• Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
• Cell movement assays (culture plates were coated with fibronectin)
• Cell spreading and immunofluorescence
• Protein specificity
• Integration of sensor films into microfluidic chips
• Histological and Immunohistochemical (IHC) analyses
• Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Domains of fibronectin bind with collagen, fibrin, heparin, and specific cell membrane receptors. The most notable domain, Arg-Gly-Asp can be recognized by integrins and mediates cell adhesion. Fibronectin is also widely involved in interactions such as the attachment and migration of many cell types, cytoskeletal assembly, tyrosine phosphorylation, and metastasis.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

Peer-Reviewed Papers
15

References

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