Filipin III from Streptomyces filipinensis ≥85% (HPLC)

Properties

Related Categories	Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Type, Enzymes, Inhibitors, and Substrates, Substrate Analogs More...
Quality Level	PREMIUM
assay	≥85% (HPLC)
storage temp.	−20°C

Description

Biochem/physiol Actions

Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols.⁴ Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.⁵

Other Notes

Filipin III is the major component of 8 isomeric components in filipin.

Application

Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel¹ and immobilized on nitrocellulose membranes.² It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.³

Price and Availability

Related Papers

1. Filipin staining of lipoproteins in polyacrylamide gels: sensitivity and photobleaching of the fluorophore and its use in a double staining method. Smejkal, G.B., and Hoff, H.F. Electrophoresis 15, 922-5, (1994)

Documents

Certificate of Analysis

F4767 - Product Information Sheet (29 KB)

Safety Information

Symbol	GHS07
Signal word	Warning
Hazard statements	H315-H319-H335
Precautionary statements	P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes (Europe)	Xi
Risk Statements (Europe)	36/37/38
Safety Statements (Europe)	26-36
WGK Germany	3
RTECS	RN9800000

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Papers

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1. Filipin staining of lipoproteins in polyacrylamide gels: sensitivity and photobleaching of the fluorophore and its use in a double staining method. Smejkal, G.B., and Hoff, H.F. Electrophoresis 15, 922-5, (1994)

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2. Cholesterol specific probe for lipoproteins immobilized on nitrocellulose membranes. Smejkal, G.B., and Hoff, H.F. Biotechniques 16, 68-70, (1994)

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3. Staining of cholesterol with the fluorescent antibiotic filipin Börnig, H., and Geyer, G. Acta Histochem. 50, 110-115, (1974)

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4. Sterol control of the phosphatidylethanolamine-phosphatidylcholine conversion in the yeast mutant GL7. Kawasaki, S., et al. Proc. Natl. Acad. Sci. U. S. A. 82, 5715-9, (1985)

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5. Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release. Marella, M., et al. J. Biol. Chem. 277, 3737-47, (2002)

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Ligand-Dependent Recruitment of the ErbB4 Signaling Complex into Neuronal Lipid Rafts Ma, L., et al. J. Neurosci. 23, 3164-75, (2003)

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CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells Schabath, H., et al. J. Cell Sci. 119, 314-25, (2006)

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Francisella targets cholesterol-rich host cell membrane domains for entry into macrophages. Tamilselvam, B., and Daefler, S. J. Histochem. Cytochem. 180, 8262-71, (2008)

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Lipid asymmetry in plant plasma membranes: phosphate deficiency-induced phospholipid replacement is restricted to the cytosolic leaflet Tjellstrom, H., et al. FASEB J. 24, 1128-38, (2009)

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Intracellular trafficking of hyaluronic acid-chitosan oligomer-based nanoparticles in cultured human ocular surface cells. Laura Contreras-Ruiz et al Mol. Vis. 17, 279-90, (2011)

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Blebbishields, the emergency program for cancer stem cells: sphere formation and tumorigenesis after apoptosis Jinesh, G.G., et al. Cell Death Differ., (2012)

Merck 14,4080