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F4771 Sigma

Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse

clone 1B10, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
biological source   mouse
antibody form   ascites fluid
clone   1B10, monoclonal
mol wt   antigen mol wt 43-72-80 kDa
contains   15 mM sodium azide
species reactivity   human
application(s)   complement-mediated cytotoxicity assay: 1:500
  flow cytometry: suitable
  immunohistochemistry (frozen sections): suitable
isotype   IgM
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C


Antigen Background

This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.

Analysis Note

Working dilution is at least 1:500 by complement-mediated microcytotoxicity assay.

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cultured human thymic fibroblasts


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.

General description

Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes. Monoclonal anti-fibroblast surface protein antibody can be used in immunohistological staining of frozen sections of human tissue. It is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. Monoclonal anti-human fibroblast surface protein antibody reacts specifically with surface protein of human fibroblasts and fibroblast cell lines. It has also shown reactivity for tissue macrophages and 95% of peripheral blood monocytes.

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Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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