EMAIL THIS PAGE TO A FRIEND

F4799 Sigma

3X FLAG® Peptide

lyophilized powder

Synonym: Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

Properties

Related Categories FLAG Affinity Purification, FLAG Peptides, FLAG System, Molecular Biology, Plant Biotechnology,
form   lyophilized powder
concentration   (Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.)
shipped in   wet ice
storage temp.   2-8°C

Description

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Application

For use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography.

Browse additional application references in our FLAG® Literature portal.

General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


New TruPAGE™ Pre-cast Polyacrylamide Gels

Customers Also Viewed

purified immunoglobulin, buffered aqueous glycerol solution

Anti-FLAG<SUP>®</SUP> M2 Magnetic Beads

affinity isolated antibody

Frequently Viewed from Other Substances

affinity isolated antibody, buffered aqueous solution

FLAG<SUP>®</SUP> Peptide

lyophilized powder

clone M2, purified immunoglobulin, buffered aqueous solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?