• USA Home
  • F4883 - Fibrinogen from human plasma

EMAIL THIS PAGE TO A FRIEND
F4883 Sigma

Fibrinogen from human plasma

35-65% protein (≥90% of protein is clottable).

Synonym: Factor I

Purchase

Properties

Related Categories Application Index, Biochemicals and Reagents, Blood, Cell Biology, Cell Signaling and Neuroscience,
Quality Level   PREMIUM
description   essentially plasmin(ogen) free
quality   35-65% protein (≥90% of protein is clottable).
mol wt   α-chain mol wt 63.5 kDa
  β-chain mol wt 56 kDa
  γ chain mol wt 47 kDa (about 4% carbohydrate content)
  soluble dimer mol wt 340 kDa
solubility   0.9% NaCl: soluble
storage temp.   −20°C
Gene Information   human ... FGA(2243), FGB(2244), FGG(2266)

Description

Analysis Note

Protein determined by biuret

Packaging

1, 5 g in glass bottle

500 mg in glass bottle

Physical form

Lyophilized powder containing sodium citrate and sodium chloride.

Application

Fibrinogen from human plasma has been used for-
• fibrinogen adsorption assay performed on swCNTs (single-walled carbon nanotubes)
• the characterization of fibrinogen structure in the presence of CNT using CD (Circular dichroism) spectroscopy and temperature scan
• the preparation of RBC suspension obtained from venous blood of antecubital vein, to assess RBC aggregability
• fibrinolysis assay performed on mouse embryonic fibroblasts (MEFs)
• the preparation of fibrinogen-labeled gold (Fgn/Au)
• the quantification of in vitro angiogenesis by human microvascular endothelial cells and
• the assessment of fibrin-induced apoptosis and differentiation-regulation in trophoblast obtained from term human placentas.

Biochem/physiol Actions

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Fibrinogen is an acute-phase inflammatory protein, which plays an essential role in blood clotting. Coronary heart disease is linked to the plasma levels of fibrinogen in Chinese population, and might have potential as a marker and risk factor for the same. A -455 allele of fibrinogen β (FGB) chain is linked with poor survival in Caucasian women with Finnish stroke. Ischemic stroke preceded by hyperfibrinogenemia has poor outcome, which is independent of the baseline fibrinogen concentration.

General description

Fibrinogen was the first blood coagulation factor to be identified, and it is a precursor of fibrin protein. This protein is composed of two chains, each of which is composed of three chains- α, β and γ. These three subunits are coded by three genes located in the human chromosomal locus 4q23-q32, and spans 51kb.

Specifications

Source material has tested negative for HIV and HBsAg.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?