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F4892 Sigma

D-Fructose Dehydrogenase from Gluconobacter sp.

lyophilized powder, ≥20 units/mg solid

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
form   lyophilized powder
mol wt   mol wt ~140 kDa
shipped in   wet ice
storage temp.   −20°C

Description

Packaging

250 units in glass bottle

Unit Definition

One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.

Physical form

supplied as a lyophilized powder containing approx 80% stabilizers, sugars, amino acids and BSA

Application

This enzyme is also used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.This enzyme is useful for enzymatic determination of D-fructose in clinical analysis.

Biochem/physiol Actions

D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

Physical properties

Isoelectric point : 5.0 ± 0.1
Michaelis constant : 5 x 10‾3M (D-Fructose)
Inhibitors : Ag+, Hg++, SDS
Optimum pH : 4.0
Optimum temperature : 37°C
pH Stability : pH 4.0 – 6.0 (25°C, 16hr)
Thermal stability : Below 40°C (pH 4.5, 15min)

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Assay Procedure for D-Fructose Dehydrogenase

Prussian blue : KFeⅢ[FeⅡ(CN)6] The appearance of prussian blue formed by chelate reaction is measured at 660nm by spectrophotometry.
Keywords: Extinction coefficient, Oxidations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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