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  • F5012 - Anti-Ferritin, Human antibody produced in rabbit

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F5012 Sigma

Anti-Ferritin, Human antibody produced in rabbit

whole antiserum, liquid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, Cancer Markers And Antibodies Antibodies, Cancer Markers and Antibodies,
species reactivity   human
application(s)   Ouchterlony double diffusion: 1:16
clone   polyclonal
antibody form   whole antiserum
form   liquid
contains   15 mM sodium azide
storage temp.   −20°C
Gene Information   human ... FTH1(2495)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Ferritin isolated from normal human spleen

General description

Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum.

Application

Anti-ferritin, human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen extract), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C).

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