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F5022 Sigma

Fibronectin-like Protein Polymer genetically engineered

lyophilized powder, autoclaved, BioReagent, suitable for cell culture

Synonym: ProNectin® F

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Properties

Related Categories Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture, Core Bioreagents,
sterility   autoclaved
product line   BioReagent
form   lyophilized powder
mol wt   mol wt 75 kDa by gene sequence (differences are due to the unusual amino acid composition of the protein)
  mol wt ~110 kDa by SDS-PAGE
solubility   4.5  M LiClO4: 1 mg/mL (stable for 12 mo. at RT.)
suitability   suitable for cell culture

Description

Application

Recommended as a cell culture substratum at 2-10 μg/cm2.

Other Notes

Positively-charged, protein polymer which incorporates multiple copies of the RGD cell attachment epitope from human fibronectin between repeated structural peptide units.

Packaging

Supplied with diluent in separate vial.

Reconstitution

Dilutions can be made to a working concentration with balanced salt solution; stable up to 2 days at RT. Coated labware is stable at least 4 mos at RT.

Legal Information

ProNectin is a registered trademark of Sanyo Chemical Industries, Ltd

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

Fibronectin (FN)

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

Peer-Reviewed Papers
15

References

Related Products

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