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F5027 Sigma

Monoclonal Anti-IgD−FITC antibody produced in mouse

clone HJ9, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Human IgD

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Anti-Immunoglobulins, Antibodies, Antibodies for Cell Biology,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   HJ9, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... IGHD(3495)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

human IgD

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity

Recognizes the delta chain of human IgD and is not reactive with heavy chains from human IgG1, IgG2, IgG3, IgG4, IgM, IgA, or kappa and lambda light chains.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Application

Monoclonal Anti-IgD-FITC antibody is suitable for flow cytometry at a concentration of 10μL using 1×106 cells.

Other Notes

IgD is expressed on peripheral B cells and is used as a pan-B cell marker.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

IgD is the major antigen receptor isotype coexpressed with IgM on the surface of human peripheral B cells. It is associated with early antibody response. It occupies approximately 0.25% of total immunoglobulins. In serum, it has shown that the catabolism of IgD is decreased at high concentrations. IgD molecule comprises of a long “hinge” region (between Fab and Fc) including three constant region domains. This makes the molecule very susceptible to proteolytic degradation with production of Fab and Fc fragments. It also helps to maintain the molecule flexibility that enhance antigen binding capacity. IgD is a potent inducer of tumor necrosis factor (TNF), Interlukin 1β(IL1β), and Interlukin IL1RN (IL1RN). It also accelerates the release of IL6, IL10 from peripheral blood mononuclear cells. Its increased level in serum causes hyper-IgD and periodic fever syndrome (HIDS).

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Certificate of Analysis

Safety Information

WGK Germany  3
Protocols & Articles

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Peer-Reviewed Papers
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