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F5273 Sigma

Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Rat Kappa & Lambda Light Chains

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Anti-Immunoglobulins, Antibodies, FITC Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   RT-39/RL-6, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa
storage condition   protect from light
application(s)   dot immunobinding: 1:40
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
isotype   IgG1/IgG2a
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

General description

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

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Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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