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  • F5273 - Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

F5273 Sigma

Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Rat Kappa & Lambda Light Chains



Related Categories Alphabetical Index, Analytical Cytology, Anti-Immunoglobulins, Antibodies, FITC Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   RT-39/RL-6, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa
storage condition   protect from light
application(s)   dot immunobinding: 1:40
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
isotype   IgG1/IgG2a
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C


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Physical form

Solution in 0.01 M phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

General description

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

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Safety Information

NONH for all modes of transport
WGK Germany 
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