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  • F5421 - Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit

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F5421 Sigma

Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FGFR-1

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using trypsin-digested human and animal tissue sections
  immunoprecipitation: suitable
  western blot: 1:400 using an extract of FGFR-1 transfected cells
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 110-120 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FGFR1(2260)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to amino acid residues 360-373 of the extracellular region of human FGFR-1 conjugated to KLH with glutaraldehyde, and with a C-terminally added lysine.

General description

Fibroblast growth factor receptors (FGFR) are tyrosine kinases that regulate growth and development. FGFRs have been classified in to four main types, ranging from FGFR-1 to FGFR-4. FGFR-1 amplifications have been associated with various cancers such as non-small cell lung cancer, breast cancer and urothelial carcinomas . Mutations in FGFR-1 gene have been linked to human gonadotropin-releasing hormone deficiency disorders and Pfeiffer syndrome . Anti-FGFR-1 antibody is specific for human FGFR-1 and does not react with human FGFR-2 and FGFR-3. The immunizing peptide specifically inhibits the staining of anti-FGFR-1 antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Anti-Fibroblast Growth Factor Receptor-1 (FGFR-1) is developed in rabbit using a synthetic peptide Glu-AlaLeu-Glu-Glu-Arg-Pro-Ala-Val-Met-Thr-Ser-Pro-Leu-Lys conjugated to KLH with glutaraldehyde as immunogen. The peptide corresponds to amino acid residues 360-373 of the extracellular region of human FGFR-1 with a C-terminally added lysine.
Staining of the product is specifically inhibited with the FGFR-1 immunizing peptide. No reaction with human FGFR-2 and FGFR-3 is detected.

No reaction with FGFR-2 (Bek) and FGFR-3 is detected.

Application

Anti-FGFR-1 may be used for immunoblotting (approximately 110 kDa and 120 kDa), immunoprecipitation, and immunohistochemistry.
A minimum working dilution of 1:400 is determined by immunoblotting using an extract of 293T cells transiently transfected with the pcDNA3/FGFR-1 plasmid.
A minimum working dilution of 1:100 is determined by indirect immunoperoxidase staining of trypsin-digested, formalin-fixed, paraffin-embedded human and animal tissue sections.
The epitopes recognized by the antibody are resistant to routine formalin-fixation and paraffin embedding, and to other fixatives e.g. Methacarn, Bouins solution, ethanol, and B5.

Anti-Fibroblast Growth Factor Receptor-1 antibody is also suitable for use in western blot (1:400, using an extract of FGFR-1 transfected cells).

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