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F5636 Sigma

Monoclonal Anti-FITC antibody produced in mouse

clone FL-D6, ascites fluid

Synonym: Monoclonal Anti-FITC

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Fluorescent Labels, Biochemicals and Reagents, FB-FJ,
biological source   mouse
antibody form   ascites fluid
clone   FL-D6, monoclonal
contains   15 mM sodium azide
application(s)   indirect ELISA: 1:2,500 using FITC-conjugate
  indirect immunofluorescence: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

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Immunogen

FITC-BSA conjugate

Specificity

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Application

Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It may be used in ELISA, competitive ELISA and immunofluorescent techniques. A FITC Anti-FITC system has been used in the amplification of signal in immunofluorescent detection and as a means of separating bound from free tracer by affinity chromatography. The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface.

Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).

General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
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