• USA Home
  • F5636 - Monoclonal Anti-FITC antibody produced in mouse

F5636 Sigma

Monoclonal Anti-FITC antibody produced in mouse

clone FL-D6, ascites fluid

Synonym: Monoclonal Anti-FITC



Related Categories Alphabetical Index, Antibodies, Antibodies to Fluorescent Labels, Biochemicals and Reagents, FB-FJ,
biological source   mouse
antibody form   ascites fluid
clone   FL-D6, monoclonal
contains   15 mM sodium azide
application(s)   indirect ELISA: 1:2,500 using FITC-conjugate
  indirect immunofluorescence: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C


Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.


FITC-BSA conjugate


The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Immunofluorescence (1 paper)

Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It may be used in ELISA, competitive ELISA and immunofluorescent techniques. A FITC Anti-FITC system has been used in the amplification of signal in immunofluorescent detection and as a means of separating bound from free tracer by affinity chromatography. The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface.

Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).

General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?