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F5766 Sigma

Monoclonal Anti-Folic Acid antibody produced in mouse

clone VP-52, ascites fluid

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Properties

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biological source   mouse
antibody form   ascites fluid
clone   VP-52, monoclonal
contains   15 mM sodium azide
application(s)   indirect ELISA: 1:500
isotype   IgG2b
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

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Immunogen

5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH

Application

Monoclonal Anti-Folic Acid antibody is suitable for use in western blot (diluted 1:1000).

General description

Folic acid (folate) is a vitamin that regulates one-carbon transfer during the conversion of homocysteine to methionine. Methionine is a precursor of S-adenosylmethionine, which is the main methyl donor for methylation reactions. Thus, folic acid is essential for regulation of DNA methylations and for epigenetic modifications that modulate gene expression. Folate deficiency has been associated with a wide range of disorders including colorectal cancers, depression, neural tube defects and cerebral dysfunctions
Monoclonal Anti-Folic Acid antibody binds to folate and detects an epitope present on both folic acid and its biologically active analog 5-methyltetrahydrofolic acid (5MTHFA). The product reacts with free folate, folate conjugated to a carrier such as KLH or BSA, and folic acid in human plasma or serum bound to the endogenous Folate Binder. The antibody does not cross-react with tetrahydrofolic acid, folinic acid, dihydrofolic acid or vitamin (B12).

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What immunogen was used to generate Product No. F5766, Monoclonal Anti-Folic Acid antibody?
The immunogen used was 5-Methyltetrahydrofolic acid (sometimes called 5MTH folic acid) conjugated to keyhole limpet hemocyanin (KLH).
What is the name of the antibody clone for Product No. F5766, Monoclonal Anti-Folic Acid antibody?
The clone is VP-52.
What is the concentration of Product No. F5766, Monoclonal Anti-Folic Acid antibody?
The concentration of the antibody is lot specific and can be found on the certificate of analysis. (It is typically in the 1-2 mg/mL range).
What is the isotype of Product No. F5766, Monoclonal Anti-Folic Acid antibody?
This antibody is mouse IgG2B.
Will Product No. F5766, Monoclonal Anti-Folic Acid antibody, work in Western blotting?
The use of this antibody for western blotting has been reported in one study on Pseudomonas and tumor cells. See: Leamon, C.P., et al., Cytotoxicity of Folate-Pseudomonas Exotoxin Conjugates Toward Tumor Cells. J. Biol. Chem., 268(33), 24847-24854 (1993). The antibody was used at a 1 to 1000 dilution in Tris buffered saline (TBS) for 1 hour at 23°C.
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Protocols & Articles

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Peer-Reviewed Papers
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References

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