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F5900 Sigma

Monoclonal Anti-Interleukin-2−Carboxyfluorescein antibody produced in mouse

clone 5334, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-IL-2, Monoclonal Anti-Interleukin-2

Description

Immunogen

recombinant human IL-2

General description

Interleukin-2 (IL-2) also refers as T Cell Growth Factor is a cytokine produced by certain subsets of T lymphocytes. It plays various functions like inducing interferon γ and B cell growth factor secretion as well as affecting the activation and proliferation of NK cells. It also has a vital role in modulating the expression of the IL-2 receptor and can facilitate B cell growth and differentiation. Monoclonal anti-Interleukin-2−Carboxyfluorescein antibody reacts specifically with human IL-2.

Physical form

Solution in phosphate buffered saline containing 0.1% sodium azide.

Specificity

The conjugates will identify and quantitate cells containing intracellular forms of human IL-2 by flow cytometry.

Preparation Note

Purified antibody is conjugated to carboxyfluorescein.

Application

Monoclonal anti-Interleukin-2−Carboxyfluorescein antibody can be used in flow cytometry assays for identifying intracytoplasmic cytokines and for enumerating cytokine producing cells present in a mixed population.

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