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F6005 Sigma

Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, Primary Antibodies, RAB-RAD,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   indirect immunofluorescence: 1:40
conjugate   FITC conjugate
storage temp.   2-8°C

Description

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Immunogen

Purified rabbit IgG

Packaging

1, 2 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-Rabbit IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:40. The antibody was used for immunohistochemistry of dorsal root ganglia neurons of chicken embryos at a working antibody dilution of 1:500 using anti-neurofilament-M as primary antibody.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Rabbit IgG (whole molecule)-FITC antibody is specific for all rabbit IgG subclasses. Goat Anti-Rabbit IgG is conjugated to Fluorescein Isothiocyanate (FITC) in an alkaline reaction, further purified to remove unbound FITC.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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Peer-Reviewed Papers
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References

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