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F6131 Sigma

Fetuin from fetal bovine serum

γ-irradiated, BioXtra, suitable for cell culture

Synonym: Fetuin from fetal calf serum

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Properties

Related Categories Albumins and Transport Proteins, Cell Culture, Miscellaneous Plasma Proteins, Reagents and Supplements
sterility   γ-irradiated
product line   BioXtra
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Analysis Note

This product is cell culture tested (0.5 g/L) and is appropriate for use in cell culture applications.

General description

Fetuin is a protein component of serum which contributes to the attachment and spreading of cells in culture medium. Researchers often add serum for this purpose. Fetuin has been used at a concentration of 500 μg/ml to supplement serum-free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells. Fetuin can be used as a glycoprotein standard for carbohydrate structure in a glycoprotein. The structural elucidation of the carbohydrate portion of fetuin has been published. The cDNA and amino acid sequences has been reported. Fetuin contains 12 Cys residues, all involved in disulfide bridges.

The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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