• USA Home
  • F6140 - Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
F6140 Sigma

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

clone FN-3E2, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   human, chicken, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect immunofluorescence: 1:400 using cultured chicken fibroblasts
  radioimmunoassay: suitable
  western blot: suitable
clone   FN-3E2, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 240 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FN1(2335)
mouse ... Fn1(14268)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

fibronectin antigen released in culture by a human breast cancer cell line

General description

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis . Monoclonal anti-fibronectin, cellular antibody is specific for fibronectin in the pericellular matrix of cultured chicken fibroblasts. The antibody also labels cellular fibronectin in chicken embryo, mouse and human fibroblasts.

Application

Monoclonal Anti-Fibronectin, Cellular antibody is suitable for use in immunohistochemistry (using formalin-fixed, paraffin-embedded sections or using frozen sections), immunoprecipitation, radioimmunoassay, western blot and indirect immunofluorescence (1:400 using cultured chicken fibroblasts).The antibody can also be used for immunoperoxidase or immunofluorescent labeling in frozen human tissues and for immunoaffinity purification of cellular fibronectin. In immunoblotting, monoclonal anti-cellular fibronectin localizes at 240 kDa bands (using native or denatured forms of cellular fibronectin).

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Price and Availability


Detect Your Unseen Protein

All labs need water

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?