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  • F6140 - Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

F6140 Sigma

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

clone FN-3E2, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   human, chicken, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect immunofluorescence: 1:400 using cultured chicken fibroblasts
  radioimmunoassay: suitable
  western blot: suitable
clone   FN-3E2, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 240 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FN1(2335)
mouse ... Fn1(14268)
biological source   mouse
conjugate   unconjugated



fibronectin antigen released in culture by a human breast cancer cell line

General description

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis . Monoclonal anti-fibronectin, cellular antibody is specific for fibronectin in the pericellular matrix of cultured chicken fibroblasts. The antibody also labels cellular fibronectin in chicken embryo, mouse and human fibroblasts.


Monoclonal Anti-Fibronectin, Cellular antibody is suitable for use in immunohistochemistry (using formalin-fixed, paraffin-embedded sections or using frozen sections), immunoprecipitation, radioimmunoassay, western blot and indirect immunofluorescence (1:400 using cultured chicken fibroblasts).The antibody can also be used for immunoperoxidase or immunofluorescent labeling in frozen human tissues and for immunoaffinity purification of cellular fibronectin. In immunoblotting, monoclonal anti-cellular fibronectin localizes at 240 kDa bands (using native or denatured forms of cellular fibronectin).

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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