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  • F6162 - Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse

F6162 Sigma

Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse

clone FB-8, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-FGF2


Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human, bovine
application(s)   dot blot: suitable
  indirect ELISA: suitable
  neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells
  radioimmunoassay: suitable
  western blot: suitable
clone   FB-8, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 18 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FGF2(2247)
biological source   mouse
conjugate   unconjugated



recombinant human FGF2.

General description

The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4.


Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse was used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture.

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Biochem/physiol Actions

Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis.

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