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F6257 Sigma

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   sheep
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct immunofluorescence: 1:32
conjugate   FITC conjugate
storage temp.   2-8°C

Description

Features and Benefits

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Immunogen

Purified mouse IgG

Packaging

0.5 mL in glass insert

1, 2 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:32. For immunofluorescence of human ovarian adenocarcinoma cells stained with anti-integrin primary antibodies a working dilution of 1:50 was used. Immunolocalization of human spermatids was done using antibody dilution of 1:100. This antibody was also used for immunofluorescent tagging of leaf cells of Arabidopsis.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for all mouse IgG subclasses. Affinity isolated antigen specific antibody is obtained from sheep anti-mouse IgG antiserum by immunospecific purification. The antibody preparation is then conjugated to Sigma Fluorescein Isothiocyanate (FITC), Isomer I.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
Keywords: Cancer, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatography, Clinical, Degradations, Electrophoresis, Enzyme-linked immunosorbent assay, Gene expression, High performance liquid chromatography, Immunohistochemistry, Immunoprecipitation, Liquid chromatography mass spectrometry, Mass spectrometry, Mass spectrometry detection, Pharmaceutical, Purification, Sequencing, Titrations, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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Peer-Reviewed Papers
15

References

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