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F6397 Sigma

IgG1−FITC Isotype Control from murine myeloma

clone MOPC 21, purified immunoglobulin, buffered aqueous solution

Synonym: IgG1 Isotype Control from murine myeloma

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Properties

Related Categories Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls,
biological source   mouse
antibody form   purified immunoglobulin
clone   MOPC 21, monoclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   flow cytometry: suitable
conjugate   FITC conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

IgG1-FITC Isotype Control from murine myeloma was used as negative control in FACS of human erythroid cell population that is expresses CD17.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

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Peer-Reviewed Papers
15

References

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