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F6522 Sigma

IgG2a−FITC Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls,
biological source   mouse
antibody form   purified immunoglobulin
clone   UPC-10, monoclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   flow cytometry: suitable
conjugate   FITC conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

IgG2a-FITC isotype control antibodies can be used for immunostaining (using hepatic stellate cell cultures from rats ) and flow cytometric assays.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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