• USA Home
  • F6522 - IgG2a−FITC Isotype Control from murine myeloma

F6522 Sigma

IgG2a−FITC Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution



Related Categories Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls,
biological source   mouse
antibody form   purified immunoglobulin
clone   UPC-10, monoclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   flow cytometry: suitable
conjugate   FITC conjugate
shipped in   wet ice
storage temp.   2-8°C


Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


IgG2a-FITC isotype control antibodies can be used for immunostaining (using hepatic stellate cell cultures from rats ) and flow cytometric assays.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?