|Related Categories||Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Clone Index,|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|storage condition||protect from light|
|application(s)||flow cytometry: suitable|
|shipped in||wet ice|
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
IgG2a-FITC isotype control antibodies can be used for immunostaining (using hepatic stellate cell cultures from rats 1) and flow cytometric2 assays.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases 3. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice 4
Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.
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Certificate of Analysis
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
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