IgG2a−FITC Isotype Control from murine myeloma clone UPC-10, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories	Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Clone Index, Isotype Controls, Mouse Isotype Controls, Primary Antibodies, Proteins and Derivatives, U More...
antibody form	purified immunoglobulin
clone	UPC-10, monoclonal
form	buffered aqueous solution
storage condition	protect from light
application(s)	flow cytometry: suitable
shipped in	wet ice
storage temp.	2-8°C

Description

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

IgG2a-FITC isotype control antibodies can be used for immunostaining (using hepatic stellate cell cultures from rats ¹) and flow cytometric² assays.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases ³. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice ⁴
Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.

Price and Availability

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