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F6550 Sigma

Anti-FLIPα, C-Terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-CASH, Anti-CLARP, Anti-Casper, Anti-FLAME-1, Anti-I-FLICE

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Suppressors of Apoptosis,
species reactivity   human
application(s)   western blot: 0.5 μg/mL using human HeLa cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CFLAR(8837)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 447-464 of human FLIPα/FLIPL.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide

Specificity

By immunoblotting, the antibody detects human FLIPα (FLICE-inhibitory protein).

Application

Anti-FLIPα, C-Terminal antibody is suitable for:
• western blotting of whole L540 cell extracts to detect c-flip activity in the regulation of NF-κB distribution and activity by 5F11 and bortezomib
• immunohistochemistry analysis to study the optimization of c-FLIP activity in lymphoma cells
• immunohistochemical cytoplasmic c-Flip staining to detect c-FLIP expression in Burkitt′s lymphoma (BL) may be a reliable prognostic marker
• western blot analysis at a concentration of 0.5μg/mL using human HeLa cell extract.

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Safety Information

WGK Germany  3
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Peer-Reviewed Papers
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