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F6796 Sigma

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FGFR-2

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections
  western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 115-125 kDa (doublet)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FGFR2(2263)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

General description

Fibroblast growth factors (FGFs) are members of a large tyrosine kinase family of growth factor receptors (17-38kDa) that are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted8 or possibly cleaved9 forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity
Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

Fibroblast growth factors (FGFs) are members of a large tyrosine kinase family of growth factor receptors (17-38kDa) that are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted8 or possibly cleaved9 forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid ‘acidic box’, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity
Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline,
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity

The antibody detects human FGFR-2 (bek) in extracts of transfected cells.

The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.

Preparation Note

Affinity isolated on a peptide-agarose column.

Application

Immunoblotting: a minimum working antibody dilution of 1:2,000 is determined using a whole extract of transfected cells expressing recombinant human FGFR-2. Immunohistochemistry: a minimum working dilution of 1:1,000 is determined using indirect immunoperoxidase staining and trypsin-digested, formalin-fixed, paraffinembedded human and animal tissue sections.

Features and Benefits

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Packaging

0.2 mL in glass bottle

Price and Availability


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