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F7250 Sigma

Fluorescein isothiocyanate isomer I

suitable for protein labeling, ≥90% (HPLC), powder

Synonym: FITC, Fluorescein 5-isothiocyanate



Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.

Fluorescein isothiocyanate isomer I is suitable reagent for protein labeling studies. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.


1, 5 g in glass bottle

50, 100, 250, 500 mg in glass bottle

General description

Fluorescein isothiocyanate isomer I (FITC) has been proposed as a contact sensitizer.

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


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