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F7264 Sigma

Fixing solution

5 × (solution)

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Properties

Related Categories Fixing Reagents, Molecular Biology, Protein Electrophoresis, Protein Stains and Staining Reagents, Proteomics More...
concentration   5 × (solution)

Description

Other Notes

60% (w/v) trichloroacetic acid, 17.5% (w/v) 5-sulfosalicylic acid.

Application

An effective protein fixing solution for pre-stain fixing, suitable for SDS-PAGE, non-denaturing PAGE and IEF. Proteins require fixing into the gel for analytical purposes so that they do not leach out following electrophoresis.

Price and Availability


Tools for Large Molecule BioAnalysis

Detect Your Unseen Protein
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2564 8 / PGII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Protocols

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximu...
Keywords: Electrophoresis, Isoelectric focusing, PAGE

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