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F7264 Sigma

Fixing solution

5 × (solution)

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Properties

Related Categories Fixing Reagents, Molecular Biology, Protein Electrophoresis, Protein Stains and Staining Reagents, Proteomics More...
concentration   5 × (solution)

Description

Other Notes

60% (w/v) trichloroacetic acid, 17.5% (w/v) 5-sulfosalicylic acid.

Packaging

1 L in poly bottle

Application

An effective protein fixing solution for pre-stain fixing, suitable for SDS-PAGE, non-denaturing PAGE and IEF. Proteins require fixing into the gel for analytical purposes so that they do not leach out following electrophoresis.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2564 8 / PGII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Protocols

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximu...
Keywords: Electrophoresis, Isoelectric focusing, PAGE

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