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F7425 Sigma

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ddddk, Anti-dykddddk

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO,
biological source   rabbit
antibody form   affinity isolated antibody
form   buffered aqueous solution
shelf life   1 mo. at 2 - 8 °C
concentration   ~.8 mg/mL
application(s)   dot blot: 1-2.5 μg/mL
  immunoprecipitation: 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
  indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
  western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein
immunogen sequence   DYKDDDDK
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

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General description

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

Immunogen

FLAG; peptide sequence DYKDDDDK

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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