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F7425 Sigma

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ddddk, Anti-dykddddk

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO,
biological source   rabbit
antibody form   affinity isolated antibody
form   buffered aqueous solution
shelf life   1 mo. at 2 - 8 °C
concentration   ~.8 mg/mL
application(s)   dot blot: 1-2.5 μg/mL
  immunoprecipitation: 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
  indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
  western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein
immunogen sequence   DYKDDDDK
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

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General description

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

Immunogen

FLAG; peptide sequence DYKDDDDK

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?
F7425 is a polyclonal antibody from rabbit.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/sigma/datasheet/F7425dat.pdf
What is the concentration of Product F7425, ANTI-FLAG® from rabbit?
The concentration is 0.9 mg protein per ml.
How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?
For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 µg/ml concentration should be used when using thie antibody in an ELISA application.
How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?
To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.
Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?
K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708. (http://www.idealibrary.com/links/doi/10.1006.bbrc.1995.1245) http://www.jci.org/cgi/reprint/103/8/1211     
What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?
The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975More information can be found on the positive controls by clicking: http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/P7582 http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/P7457 http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/P5975
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Peer-Reviewed Papers
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References

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06693 Timestrip Plus -20 °C

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