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F7524 Sigma

Fetal Bovine Serum

non-USA origin, sterile-filtered, suitable for cell culture

Synonym: FBS, sera, serum

Purchase

Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Fetal Bovine Serum (FBS),
sterility   sterile-filtered
origin   non-USA origin
availability   available only in EU
  not available in Canada
impurities   ≤10 EU/mL endotoxin
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Endotoxin and hemoglobin tested

Features and Benefits

F7524 is the same great product as Biochrom Ltd. product S0115.

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Application

FBS is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Price and Availability


Cell Freezing

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Are all Fetal Bovine Serum (FBS) products heat inactivated?
Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Is Product F7524, Fetal Bovin Serum, available in the US or Canada?
No, Product No. F7524 is only available in Europe.
Where is Product F7524, Fetal Bovin Serum, sourced from?
This product is a European sourced serum, most of it coming from France with very few batches coming from South America.
Is Product F7524, Fetal Bovin Serum, a USDA approved serum?
This is a non-USDA approved serum. The serum is tested for BVD (bovine virus diarrhea), PI3 (parainfluenza type 3), and IBR (infectious bovine rhinotracheitis) only.
How is Product F7524, Fetal Bovin Serum, sterilized?
This product is sterilized by filtration through three (3) sterile filter membranes, each of which has a pore size of 0.1 microns.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Does Sigma-Aldrich offer delipidated bovine serum?
At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum). A method for lipid depletion, though not necessarily that of Sigma: 1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum 2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing. 3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes. 4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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