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F7776 Sigma

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)−FITC antibody produced in mouse

clone MAPK-YT, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)

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Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
species reactivity   yeast, Xenopus, human, rat, bovine, Caenorhabditis elegans, Drosophila, mouse, hamster
application(s)   direct immunofluorescence: 1:20 using cultured human foreskin fibroblasts (paraformaldehyde-fixation)
clone   MAPK-YT, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen ERK-1 mol wt 44 kDa
  antigen ERK-2 mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK3(5595)
mouse ... Mapk1(26413), Mapk3(26417)
rat ... Mapk1(116590), Mapk3(50689)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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