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F7799 Sigma

Anti-c-Fos antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colorectal carcinoma tissue
  western blot: 1:1,000 using nuclear extract of a phorbol ester-treated human A431 cells or nuclear extract of human A431 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 50-60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FOS(2353)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal region of the human c-Fos proto-oncogene (p55), amino acids 3-16.

General description

Rabbit polyclonal anti-c-Fos antibody recognizes c-Fos by immunoblotting, (as single or multiple bands at 50-62 kDa) and by immunohistochemistry. By immunoblotting, the antibody may also detect bands of ~40 kDa representing c-Fos degradation products. Staining of c-Fos by immunoblotting is specifically inhibited with c-Fos immunizing peptide (c-Fos human, amino acids 3-16 with C-terminally added lysine).

c-Fos belongs to a family of transcription factors, including FosB, Fra1, Fra2. The fos oncogene was isolated as a retroviral transforming gene (v-fos) carried by the FBJ-murine osteosarcoma virus. c-Fos undergoes post-translational modifications and other forms have been described including 57 kDa, 60 kDa and 62 kDa proteins. c-Fos plays an important role in cell proliferation and differentiation. In addition, it is involved in cellular responses to stress, cell damage and death, and has a central role in normal bone and hematopoietic cell development and in oncogenesis. c-Fos is rapidly and transiently induced in almost every cell type upon stimulation by a variety of extracellular stimuli, including stress, mitogenic growth factors, cytokines, neurotransmitters and pharmacological agents. Stable expression of c-Fos has been shown in developing bone tissue and teeth, hematopoietic cells,germ cells, and in the central nervous system. High levels of c-Fos are expressed in human full term fetal membranes. Increased expression of c-Fos is found in some human carcinomas, including colon and pancreatic adenocarcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Rabbit polyclonal anti-c-Fos antibody is used to tag c-Fos for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of c-Fos in a wide variety of cell processes including cell proliferation and differentiation, and responses to cell damage or stress.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
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References

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