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F8402 Sigma

Monoclonal Anti-CD31 (PECAM-1)−FITC antibody produced in mouse

clone WM-59, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-PECAM-1, Monoclonal Anti-CD31

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   WM-59, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 130-140 kDa
shipped in   wet ice
storage condition   protect from light
storage temp.   2-8°C
Gene Information   human ... PECAM1(5175)
biological source   mouse
conjugate   FITC conjugate

Description

Immunogen

human cell line RC-2A, originally derived from myeloid leukemia cells.

General description

CD31 is a single chain integral membrane glycoprotein member of the immunoglobulin superfamily of cell adhesion molecules with molecular mass of 130-140kDa. It has six Ig-like loops in the extracellular domain, a trans-membrane domain including a relatively long cytoplasmic domain. It is expressed abundantly on platelets, myeloid cells, B lymphocytes, certain T-lymphocyte cell subsets, bone marrow precursor cells, NK cells and localized to the intracellular junctions in monolayers of cultured endothelial cells.

Antigen Background

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody is suitable for immunostaining of HEPC-CB2 and HEPC-CB.2 cell Lines (hematopoietic cells) and flow cytometry to evaluate the percentage of contaminating endothelia cells in characterization and decidualization of endometrial stromal cells.

Features and Benefits

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Biochem/physiol Actions

CD31 performs in homophilic and heterophilic cell-cell adhesion and cell signalling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix.

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Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Peer-Reviewed Papers
15

References

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