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  • F8646 - Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

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F8646 Sigma

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
conjugate   FITC conjugate
storage temp.   −20°C

Description

Features and Benefits

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Other Notes

Antibody adsorbed with bovine, equine, and human serum proteins.

Immunogen

Purified mouse IgG

Preparation Note

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Specificity

Binds mouse IgG; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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Secondary Antibodies, Conjugates and Kits

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Peer-Reviewed Papers
15

References

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