Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody produced in goat

Features and Benefits

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Other Notes

Antibody adsorbed with bovine, equine, and human serum proteins.

Immunogen

Purified mouse IgG

Preparation Note

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Specificity

Binds mouse IgG; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein.¹ The antibody was also used to label erythrocytes for photon reassignment microscopy² and for FACS using melanoma cells.³

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections⁴
Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.