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F8648 Sigma

Monoclonal Anti-Interferon-γ−Carboxyfluorescein antibody produced in mouse

clone 25723.11, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Interferon-γ

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Intracellular Cytokine Staining, Cell Biology,
species reactivity   human
application(s)   flow cytometry: suitable
clone   25723.11, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... IFNG(3458)
biological source   mouse
conjugate   carboxyfluorescein conjugate

Description

Immunogen

recombinant human IFN-γ

General description

Interferons (IFNs) are cytokines that are secreted in response to viral or bacterial infections and tumorigenesis. There are two types of IFNs, type I (IFN-α and IFN-β) and type II (IFN-γ). IFN-γ binds to specific receptor complex consisting of IFNγR1 and IFNγR2. There are many pathways that are mediated by IFN-γ binding such as JAK/STAT1, AP-1, NF-κB, STAT3 and STAT5. IFN-γ is pleotropic and performs various functions related to immune response, inflammation, differentiation and activation of T cells, cell cycle and apoptosis. The most studied function of IFN-γ is priming the antigen presenting cells by up regulating major histocompatibility (MHC) Class I molecules. IFN-γ has clinical applications in autoimmune diseases (rheumatoid arthritis), multiple sclerosis, cancer, HIV and fungal infections. Anti-Interferon-γ recognizes intracellular human IFN-γ. The product is prepared by conjugation of carboxyfluorescein to Protein G purified IFN-γ monoclonal antibody.

Physical form

Solution in phosphate buffered saline containing 0.1% sodium azide.

Specificity

The conjugates will identify and quantitate cells containing intracellular forms of human IFN-γ by flow cytometry.

Preparation Note

Purified antibody is conjugated to carboxyfluorescein.

Application

Anti-interferon-γ antibody may be used for detection of intracellular IGN-γ in peripheral blood lymphocytes by adding 10 μL of the antibody to 1-5 X 105 cells.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Peer-Reviewed Papers
15

References

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I5521 Monoclonal Anti-Interferon-γ antibody produced in mouse, clone 25718, purified immunoglobulin, lyophilized powder

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