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F9259 Sigma

Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct immunofluorescence: 1:128
conjugate   FITC conjugate
storage temp.   2-8°C

Description

Features and Benefits

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Immunogen

Purified mouse IgM

Packaging

0.5 mL in glass insert

1, 2 mL in glass bottle

Specificity

Binds mouse IgM only; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Mouse IgM (μ-chain specific)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a minimum working antibody dilution of 1:128. It was also used for immunohistochemistry of rat heart tissue sections at a working dilution of 1:50. A dilution of 1:100 was used to label human ovarian-adenocarcinoma cells for immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Mouse IgM (μ-chain specific)-FITC antibody is specific for mouse IgM with mu chain. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM. Goat anti-mouse IgM is then conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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