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F9291 Sigma

Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse

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Description

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General description

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Preparation Note

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Physical form

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Application

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Browse additional application references in our FLAG® Literature portal.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Glycan Labeling

GlycoProfile™ Products for Metabolic Glycan Labeling FLAG® Antibodies for Detection of FLAG-labeled Glycans GlycoProfile™ Labeling Kits Fluorophores Chromophores Additional Reagents for Reductive Ami...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Aminations, Atomic absorption spectroscopy, Capillary electrophoresis, Cell proliferation, Chromatography, Degradations, Derivatizations, Electrophoresis, Gel electrophoresis, Glycosylations, High performance liquid chromatography, Ion Exchange, Mass spectrometry, Metabolism, Peptide synthesis, Phase transitions, Post translational modifications, Reductive aminations, Separation

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Peer-Reviewed Papers
15

References

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