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F9665 Sigma

Fetal Bovine Serum

Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture

Synonym: FBS

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Fetal Bovine Serum (FBS),
sterility   sterile-filtered
composition   Hemoglobin, ≤25 mg/dL
origin   non-USA origin
availability   available only in EU
impurities   ≤10 EU/mL endotoxin
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

Endotoxin and hemoglobin tested

Preparation Note

Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat inactivation also inactivates other undetermined inhibitors of cell growth in culture.

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Application

FBS is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Price and Availability


Cell Freezing

Flow Cytometry Instruments

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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