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F9762 Sigma

Anti-Human IgM (μ-chain specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, HU-HZ, Human IgM Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
storage condition   protect from light
application(s)   ANA-indirect immunofluorescence: 1:32
  direct immunofluorescence: 1:64
conjugate   FITC conjugate
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

Purified human IgM

Specificity

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Human IgM (μ-chain specific)-FITC antibody may be used for direct immunofluorescence at a minimum working dilution of 1:64 using human peripheral blood lymphocytes. A dilution of 1:32 may be used on acetone-fixed mouse liver cells for anti-nuclear antibody assay. It may also be used for agar diffusion assay.

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Human IgM (μ-chain specific)-FITC antibody specifically binds to mu-chain of human IgM. After immunospecific purification, anti-human IgM is conjugated to fluorescein isothiocyanate (FITC), in an alkaline reaction. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins. The product is also determined to have no cross reactivity with mouse or rat serum proteins.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
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Protocols & Articles

Articles

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Peer-Reviewed Papers
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References

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