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  • F9800 - Anti-FLIP, N-Terminal antibody produced in rabbit

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F9800 Sigma

Anti-FLIP, N-Terminal antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CASH, Anti-CLARP, Anti-Casper, Anti-FLAME-1, Anti-I-FLICE

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Suppressors of Apoptosis,
species reactivity   mouse, rat, human
application(s)   western blot: 0.5-1 μg/mL
clone   polyclonal
concentration   1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa (FLIPδ/FLIPS)
  antigen mol wt 35 kDa (FLIPγ)
  antigen mol wt 52 kDa (FLIPβ)
  antigen mol wt 55 kDa (FLIPα/FLIPL)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CFLAR(8837)
mouse ... Cflar(12633)
rat ... Cflar(117279)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 2-18 of the N-terminal region of FLICE-inhibitory protein (FLIP). This sequence is identical in all FLIP splice variants.

General description

In human Viral FLICE-inhibitory proteins (v-FLIPs) is identified as c-FLIP. It is composed of two death effector domains which have structural resemblance with the N-terminal half of caspase-8 and a caspase-like domain. It exist as multiple splice variants: FLIP α, β, γ and δ. Along with splice variants, it has two endogenous forms of the protein c-FLIPlong and c-FLIPshort.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Specificity

Detects all FLIP splice variants including FLIPα (FLIPL), FLIPβ, FLIPγ, and FLIPδ (FLIPS) by immunoblotting.

Application

Anti-FLIPL C-Terminal antibody is suitable for immunoblots assay to study the expression levels of elevated c-FLIP protein expression levels in rat carotid arteries five days post-balloon injury. It is also suitable for western blot at a concentration of 0.5-1μg/mL.

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Biochem/physiol Actions

C-FLIP plays an important role in apoptosis signaling pathways. It acts as proapoptotic molecule or as an anti-apoptotic molecule. It has been reported that c-FLIP can interact with both FADD and caspase-8. It prevents caspase-8 recruitment and processing through DED-DED interaction followed by CD95-induced apoptosis.

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Safety Information

RIDADR  NONH for all modes of transport
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Peer-Reviewed Papers
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References

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