Fibronectin Proteolytic Fragment from human plasma lyophilized powder, 30 kDa

Properties

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assay	>90% (SDS-PAGE)
form	lyophilized powder
impurities	HIV and HBsAg, source material tested negative
	Small proteolytic fragments, may contain traces
storage temp.	−20°C
Gene Information	human ... FN1(2335)

Description

Application

The proteolytic fragments can be used for mapping regions, functions, and activities of fibronectin.

Physical form

Lyophilized from phosphate buffered saline with sucrose as a cryoprotectant

Biochem/physiol Actions

Fibronectin fragments are obtained from plasma fibronectin by using proteolytic enzymes. These fragments are useful tools for mapping regions within the fibronection molecule, which are responsible for specific binding to collagen and for other biologically relevant functions. The N-terminal 70 kDa fragment (Product No. F 0287) is obtained by Cathepsin D digestion. This fragment binds to both gelatin and heparin. Tryptic digestion of this 70 kDa fragment yields two peptides: the N-terminal 30 kDa heparin binding fragment (Product No. F9911) and a 45 kDa gelatin binding fragment (Product No. F 0162).

Fibronectin proteolytic fragment 30 kDa, the heparin binding fragment, is the N-terminus of fibronectin. This fragment has a basic pI (8.2–8.6) and is free ofcarbohydrate moieties. The fragment is rich in cystine residues, but has no free sulfhydryl groups. The amino acid sequence of this domain is composed of five disulfide-bonded loops. This fragment binds to heparin, fibrin, and collagen. The primary physiological role of this domain appears to be the binding of fibrin

Price and Availability

Related Papers

Interaction of N-terminal fragments of fibronectin with synthetic and recombinant D motifs from its binding protein on Staphylococcus aureus studied using fluorescence anisotropy. Huff, S., et al. J. Biol. Chem. 269, 15563, (1994)

Documents

Certificate of Analysis

F9911 - Datasheet (24 KB)

Safety Information

Symbol	GHS07
Signal word	Warning
Hazard statements	H315-H319-H335
Precautionary statements	P261-P305 + P351 + P338
Hazard Codes (Europe)	Xi
Risk Statements (Europe)	36/37/38
Safety Statements (Europe)	26-36
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions

Papers

Set your institution to view full text papers.

Interaction of N-terminal fragments of fibronectin with synthetic and recombinant D motifs from its binding protein on Staphylococcus aureus studied using fluorescence anisotropy. Huff, S., et al. J. Biol. Chem. 269, 15563, (1994)

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The collagen binding domain of fibronectin contains a high affinity binding site for Candida albicans. Negre, E., et al. J. Biol. Chem. 269, 22039, (1994)

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Product #	Description	Add to Cart
F0287	Fibronectin Proteolytic Fragment from human plasma, lyophilized powder, 70 kDa	pricing

F0162	Fibronectin Proteolytic Fragment from human plasma, lyophilized powder, 45 kDa	pricing

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Fibronectin Proteolytic Fragment from human plasma

lyophilized powder, 30 kDa