F9911 Sigma

Fibronectin Proteolytic Fragment from human plasma

lyophilized powder, 30 kDa

Description

Application

The proteolytic fragments can be used for mapping regions, functions, and activities of fibronectin.

Physical form

Lyophilized from phosphate buffered saline with sucrose as a cryoprotectant

Biochem/physiol Actions

Fibronectin fragments are obtained from plasma fibronectin by using proteolytic enzymes. These fragments are useful tools for mapping regions within the fibronection molecule, which are responsible for specific binding to collagen and for other biologically relevant functions. The N-terminal 70 kDa fragment (Product No. F 0287) is obtained by Cathepsin D digestion. This fragment binds to both gelatin and heparin. Tryptic digestion of this 70 kDa fragment yields two peptides: the N-terminal 30 kDa heparin binding fragment (Product No. F9911) and a 45 kDa gelatin binding fragment (Product No. F 0162).

Fibronectin proteolytic fragment 30 kDa, the heparin binding fragment, is the N-terminus of fibronectin. This fragment has a basic pI (8.2–8.6) and is free ofcarbohydrate moieties. The fragment is rich in cystine residues, but has no free sulfhydryl groups. The amino acid sequence of this domain is composed of five disulfide-bonded loops. This fragment binds to heparin, fibrin, and collagen. The primary physiological role of this domain appears to be the binding of fibrin

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